Coding

Part:BBa_K3788001:Experience

Designed by: Rebecca Pagès   Group: iGEM21_Aix-Marseille   (2021-09-27)


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K3788001

PCR amplification of flagCry11Aa gene :

The DNA sequence of flagcry11Aa was amplified by PCR Q5 to generate fragment allowing a SLIC cloning. The expected size is 1956bp and a 2000bp band is observed. Amplification of DNAs sequences with primers was validated.

T--Aix-Marseille--DNA-SLIC-Amplification-xtoxtox.png

Figure. Verification PCR amplification of DNAs sequence and these extensions for SLIC cloning. a. Control – (Absence of DNAs, no band expected); b, c, d, e. Amplification of 6hisp20_flagcry11Aa (expected 2500 bp), flagcry11Aa (expected 1847 bp), strepcyt1Aa (expected 775 bp) and 6hisp20 (expected 568 bp); L (SMART Ladder, its size is shown on the right side).


User Reviews

UNIQ2901e6f5e87be5f1-partinfo-00000000-QINU UNIQ2901e6f5e87be5f1-partinfo-00000001-QINU